Complete 2018 BCMB20005 Summary Notes for Theory Exam

Description

Covers all lectures in detail on the following: - Pipette and Spectrophotometer Use - Gel Electrophoresis - DNA cloning, Bacterial Transformation Process - Southern/Western Blots - PCR, RT-PCR, qRT-PCR - Cloning vs. Expression Plasmids - Selection of Recombinant/Transfected Plasmid/Host - Tissue Cultures - Native Gel Electrophoresis and SDS-PAGE - Enzyme Kinetics: Basis, Graphs, and Uses - GLUT Transporters and Hexokinases - pH, pI and Fractional Charge - UV and Colorimetric Protein Assay Methods - Bacterial Expression Systems and Affinity Tags - Cell Lysis, Protein Filtration - Protein Purification: Precipitation & Chromatography - Affinity Purification and TAG Usage - Protein Purification Calculations - Insect and Yeast Expression Systems - Baculovirus as a Protein Expression Vector