UniMelb

Semester 1, 2018


Complete 2018 BCMB20005 Summary Notes for Theory Exam

28 pages

9,752 words

$29.00

2

Description

Covers all lectures in detail on the following:

- Pipette and Spectrophotometer Use
- Gel Electrophoresis
- DNA cloning, Bacterial Transformation Process
- Southern/Western Blots
- PCR, RT-PCR, qRT-PCR
- Cloning vs. Expression Plasmids
- Selection of Recombinant/Transfected Plasmid/Host
- Tissue Cultures
- Native Gel Electrophoresis and SDS-PAGE
- Enzyme Kinetics: Basis, Graphs, and Uses
- GLUT Transporters and Hexokinases
- pH, pI and Fractional Charge
- UV and Colorimetric Protein Assay Methods
- Bacterial Expression Systems and Affinity Tags
- Cell Lysis, Protein Filtration
- Protein Purification: Precipitation & Chromatography
- Affinity Purification and TAG Usage
- Protein Purification Calculations
- Insect and Yeast Expression Systems
- Baculovirus as a Protein Expression Vector