Techniques in Molecular Science Lecture Summaries Sem 1 2019 (H1 -81)

Description

Split by topic, not necessarily in the order that it was presented in the lectures, but it was a logical enough order for me. Some are stuff that was part of the reports. Topics split into: - basic techniques (pipetting, gel electrophoresis, spectrophotometry) - DNA cloning (from Lysis to Selection) - DNA analysis (RT/Q PCR, restriction mapping, DNA libraries, southern blot) - Cell biology (staining, tissue culture) - Acids and Base (amino acids, buffers, titration, Henderson Hasselbach) - Enzymes - Protein Analysis (native gel electrophoresis, SDS page, Western blot, protein assays) - Protein expression (lysis, separation, purity assessment) - Recombinant proteins ( bacteria/insect/yeast vectors, affinity tags)