ENTIRE COURSE NOTES Molecular Biology 1

Description

All lecture notes for the entire subject. Would not have been able to get a HD without these.

TOPICS:
- Revision, Elements of Gene Structure, Agarose Gel Electrophoresis
- Extraction and Purification of Nucleic Acids → RNA + Chromosomal (genetic) DNA
- Extraction and Purification of Nucleic Acids → Plasmid DNA
- Restriction Endonucleases, DNA Ligation, Transformation + Electroporation
- Molecular Cloning Strategies
- Expressing Foreign Proteins in E. coli
- DNA Sequencing Bioinformatics + Polymerase Chain Reaction
- Polymerase Chain Reaction, Applications + Site-directed Mutagenesis
- Microbial Genetics + Regulation and Control, the lac Operon
- Southern Hybridisation, Northern Blotting and Hybridisation, RT-PCR, Western Blotting and Hybridisation