BIOL1004 Cancer and Working With Genes

Description

Notes for cancer section and final 'working with genes' section of BIOL1004. The notes are detailed, clearly set out and easy to understand. All pictures are included with the relevant text. Includes the following topics: - Cancer statistics - Environmental factors that can lead to cancer - Evasion of apoptosis - Angiogenesis - Limitless replicative potential - Invasion and metastasis - Evading the immune response - Oncogenes and tumour suppressors - Cancer and the cell cycle - P53 - Examples of inherited diseases that can lead to cancer - Evolution of cancer - Cancer treatments - Viewing DNA - Separating DNA - Cutting DNA - Palindromic Sequences - Genome Sequencing - Fixing Mistakes in DNA Replication - RFLP - Pasting DNA (Ligase I and T4 DNA Ligase) - Recombinant DNA - DNA Libraries - Labels (GFP and GUS) - PCR - AFLP - Sanger Sequencing - Molecular Cloning - Cisgenics and transgenics - Heterologous Expression - Vectors (Plasmids) - BAC and HAC - Recombinant Plasmids - Competent Cells - Transforming Eukaryotes - Microinjection and gene guns - Transforming Plants - Examples of Self-Propagation - GMO plants - De-differentiation