Semester 1, 2018

Comprehensive Notes for BCMB20005 Exam (H1)

53 pages

12,099 words




Includes all topics covered in lectures:

- Pipetting technique & spectrophotometry
- UV analysis of DNA & proteins
- Basic gel electrophoresis technique, as well as native gel electrophoresis, SDS-PAGE and vertical acrylamide gel electrophoresis
- Function and application of molecular tools including restriction enzymes and DNA ligase
- Southern and Western Blot techniques
- Basic PCR technique, as well as rtPCR and qPCR (Sybr and Taqman assays)
- Process of bacterial transformation with a gene of interest via both heat shock and electroporation
- Process of bacterial selection via antibiotic resistance and blue/white selection
- Features of cloning and expression plasmids
- Construction and purpose of both genomic and cDNA libraries
- Cell biology and application of both adherent and suspension culture
- Basic Michaelis Menten enzyme kinetics and association with GLUT transporters
- Acid-base chemistry and application to proteins via fractional charge calculation
- Protein assays - Biuret, Bradford, Lowry - and their parameters and applications
- Bacterial expression systems (pET & pGEX) and expression via the lac Operon
- Purification of proteins via dialysis or ultrafiltration
- Purification of proteins via chromatography - ion exchange chromatography, size exclusion chromatography and affinity tag chromatography
- Precipitation techniques including pH, salt and immunoprecipitation
- Protein purification tables
- Other eukaryotic expression systems including baculovirus and yeast plasmids (YEp & YIp)




UniMelb, Parkville

Member since

February 2017